Which was the hardest scene to watch in The Sopranos?

Sometimes, it’s just business. They’re soldiers after all. But what scenes hit you the hardest as the most difficult to stomach? Could be the most unfair, cruel, gut-wrenching, or cinematic scene. Are there any that you don’t want to watch again?

UPDATE:

These seem to be the top 3: Melfi’s rape, Ade’s murder, Eugene’s suicide.

Honorable mentions: Tracie’s murder , Bobby’s murder, AJ’s suicide attempt.